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Preparing LB Media in Different Water Types

Yesterday it occurred to me that I couldn’t autoclave LB media made with DDW or D2O because of D-exchange. So I had to come up with a solution which prompted me to ask the world (thank you internet)....

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Starter Cultures in DDW, DI, and 30%, 60%, and 99% D2O

Tomorrow I’ll be running the same experiment I ran yesterday, only this time I did a much better job preparing the cultures and the liquid media. The broth setup can be found here. Today I initialized...

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DDW and 30% D2O starter culture analysis

Data on figshare: 24 hour growth of e. coli in DDW and D2O. Anthony Salvagno. Figshare. Retrieved 22:57, Apr 26, 2012 (GMT) hdl.handle.net/10779/6a26c1c4c7e487101f894fe53c5e8473 Setup Notes: So I...

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E. coli growth in different water types: Methods

Today I’ll be taking time points of the e. coli growth every hour in LB suspended in DI, DDW, D2O, 30% D2O, and 60% D2O. I’m going to follow my protocol from yesterday, where I blank for the water...

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24h e. coli growth in di, ddw, d2o, 30% d2o, and 60% d2o results

24h Growth of E. coli in D2O, DDW, DI, 30% D2O, and 60% D2O. Anthony Salvagno. Figshare. Retrieved 19:23, Apr 27, 2012 (GMT) hdl.handle.net/10779/5e1e8fdaeeb4d7161c1f73990d42147e

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E. coli growth in different water types data

Via figshare: E. Coli Growth in DI, DDW, D2O, 30% D2O, and 60% D2O. Anthony Salvagno. Figshare. Retrieved 23:35, Apr 27, 2012 (GMT) hdl.handle.net/10779/51fcd2f94fd7464449ee0f794642214c I’ll post some...

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A cool figure I forgot to share: E. Coli growth in Water

the e.coli data reimagined in illustrator I made this figure for my rockethub proposal and forgot to post it here in my notebook. Look how pretty it is! I made this in Adobe Illustrator and if anyone...

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Using cuvettes in the nanodrop

I did a mini-study this morning to find out what the minimum volume needed is to get an accurate reading in the nanodrop. I have 2 different cuvettes (semi-micro and micro) and I wanted to impact the...

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Yeast and E. coli Growth: What’s next?

There are several things I can do with the yeast experiments: Another but longer time trial experiment (data every hour) Time Trials in larger quantities: maybe 100ml or 50ml – I want to see if the...

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WT E. coli colony (on D2O LB agar) morphology

Yesterday I posted some pictures of E. coli colony morphologies. This was one of the colonies, but it wasn’t as developed, so today I’m adding the extra day’s growth image. WT E. coli grown on DI LB...

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